A PROTEIN INHIBITOR OF ACID DEOXYRIBONUCLEASES

A protein inhibitor of acid deoxyribonucleases.

A protein extracted and partially purified (about 100-fold) from mouse liver is able to inhibit the acid DNases from different tissues and species, whereas pancreatic DNase and E. coli endonuclease I are not inhibited. The acid DNase displays typical Michaelis-Menten kinetics in the absence of this inhibitor, but the kinetics become sigmoidal in its presence. The existence of a DNase-inhibitor ...

Characterization of Deoxyribonucleases

Increases in deoxyribonuclease activity assayed at alkaline pH can be observed in poxvirus-infected cells when native or denatured deoxyribonucleic acid (DNA) is used as substrate. The deoxyribonuclease assayable with native DNA as substrate, induced in HeLa cells by cowpoxvirus or vaccinia virus WR, can be separated from the corresponding enzyme present in normal cells by chromatography on die...

Deoxyribonucleases in Streptomyces.

The Deoxyribonucleases of Escherichia coli

Exonuclease I from Escherichiu coli (I) (previously designated as E. coZi phosphodiesterase (2)), shows a high degree of selectivity for denatured or single stranded deoxyribonucleic acid. This enzyme attacks at the 3’-hydroxyl end of a polydeoxyribonucleotide and liberates 5’-mononucleotides in a stepwise manner until the terminal dinucleotide, which is not attacked. Because of these propertie...

Regulation of virus-induced deoxyribonucleases.